Ova and Parasites, Concentrate and Permanent Smear
Also known as O and P with Permanent Stain
What it measures
- Trichrome (1)LOINC 43227-8
- Concentrate (1)LOINC 10701-1
Before the visit
Interfering substances: Bismuth, barium (wait 7-10 days), antimicrobial agents (wait 2 weeks), gallbladder dye (wait 3 weeks after procedure).
Specimen
- Specimen
- 10 grams or 10 mL fresh stool preserved in 1 single Total-Fix transport vial or 10% formalin and polyvinyl alcohol transport vials
- Minimum volume
- 5 grams or 5 mL stool • 10 mL urine (unpreserved) • 2 mL sputum or BAL
- Also accepted
- 25 mL urine in a sterile screw-cap container • 20 mL sputum or BAL in a sterile screw-cap container or with 10% formalin
- Container
- Total-Fix transport vial 10% formalin and polyvinyl alcohol transport vials Urine submitted in a sterile screw-cap container Sputum or BAL submitted in a sterile screw-cap container or with 10% formalin
- Temperature
- Stool, sputum, and BAL (preserved): Room temperature Urine, sputum, and BAL (unpreserved): Refrigerated (cold packs)
Note: Tissue samples should be sent to pathology. Stool: Place fresh stool in a single Total-Fix transport vial or 10% formalin transport vial, as well as PVA transport medium, within 30 minutes of collection. Add stool to bring the liquid level to the "fill to here" line on the vial. Mix well. Send specimen(s) at room temperature in the same shipping container. If parasite infestation is strongly suspected, collect at least 3 stool specimens every other day, since a single specimen can be negative. If Giardia is strongly suspected, please see Giardia Antigen, EIA, Stool, Test Code 8625. Urine: Urine may be submitted unpreserved for exam for Schistosoma. Collect at mid-day. Peak egg secretion occurs between noon and 3 p.m. Do not submit first morning specimen. In patients with hematuria, eggs may be found trapped in blood and mucous in the terminal portion (last-voided portion) of the urine specimen. Trichrome Stain is not performed on non-stool samples. Sputum: The specimen should be a deep expectorated sputum, preferably collected in the early morning. A 24-hour sputum collection is also acceptable. Submit in a sterile screw-cap container, unpreserved or in 10% formalin to increase stability. Trichrome Stain is not performed on non-stool samples.
Turnaround
Set up: Daily; Report available: 4 days
Billing
CPT 87177, 87209