Lipoprotein Fractionation Ultracentrifugation

What it measures

• TriglyceridesLOINC 2571-8
• Cholesterol, TotalLOINC 2093-3
• HDL CholesterolLOINC 2085-9
• LDL CholesterolLOINC 2089-1
• VLDL, CalculatedLOINC 2575-9

Before the visit

If a cholesterol measurement is to be performed along with triglycerides, but not part of a lipid panel, then the patient should be fasting 9-12 hours prior to collection. If the cholesterol is ordered as part of a lipid panel, then a fasting sample is not required. The assay manufacturer Beckman Coulter advises: N-Acetyl Cysteine (NAC), when administered in therapeutic concentrations (for the treatment of acetaminophen overdose), has been determined to interfere with assays for cholesterol and uric acid where "NAC interference may lead to falsely low results." According to Beckman Coulter, the NAC interference should be insignificant by 12 hours after completion of the initial loading dose of an IV infusion treatment regimen consisting of an initial loading dose of 150 mg/kg administered over 1 hour, a second dose of 50 mg/kg administered over 4 hours and a third dose of 100 mg/kg administered over 16 hours.

Specimen

Specimen

4 mL serum

Minimum volume

2 mL

Container

Transport tube

Temperature

Refrigerated (cold packs)

Pleural fluid is not an acceptable specimen type

Turnaround

Set up: Mon-Fri (a.m.); Report available: 7 days

Billing

CPT 82465, 83701, 84478